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Hopkins-Cole reaction
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Hopkins-Cole reaction : ウィキペディア英語版
Hopkins-Cole reaction
The Hopkins-Cole reaction, also known as the glyoxylic acid reaction, is a chemical test used for detecting the presence of tryptophan in proteins. A protein solution is mixed with Hopkins Cole reagent, which consists of glyoxylic acid. Concentrated sulfuric acid is slowly added to form two layers. A purple ring appears between the two layers if the test is positive for tryptophan. Nitrites, chlorates, nitrates and excess chlorides prevent the reaction from occurring.
==References==


抄文引用元・出典: フリー百科事典『 ウィキペディア(Wikipedia)
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